Abstract: Objective Establishing the aging model of tree shrew induced by D-galactose and providing a model reference scheme for the study of ovarian senescence. Method (1) eight young tree shrews were selected and randomly divided into control group ( n = 4) and model group ( n = 4) . (2) the model group was subcutaneously injected with D-galactose on the back of neck according to the dose of 8 g / kg for 5 months, and the ovaries were collected, frozen in liquid nitrogen and fixed with 4% paraformaldehyde. (3) the tissue structure of ovary was observed by HE staining, the expression of aging-related molecular proteins was observed by immunohistochemical staining, cell proliferation activity was observed by Ki67 staining, and apoptosis was observed by Tunel staining. Result (1) the boundary between medulla and stroma in the ovarian tissue of tree shrew in the control group was obvious, and the cells were arranged neatly, showing primitive, primary, secondary and mature follicles with a small number of atretic follicles, while in the model group, the ovarian tissue structure of tree shrew was scattered, only primitive and primary follicles were seen locally, and there were a large number of atretic follicles. (2) the expression level of aging-related marker molecules p53, P21 and P16 in the model group was significantly higher than that in the control group. (3) the activity of cell proliferation in the model group was significantly lower than that in the control group. (4) the apoptosis rate of ovarian cells in the model group was significantly higher than that in the control group. Conclusion D-galactose can make ovarian tissue structure scattered, follicle atresia, weaken cell proliferation activity, and increase apoptosis rate.

Key words: Tree shrew, ovarian senescence, follicle, apoptosis, aging marker molecule

摘要： 目的 通过 D-半乳糖诱导法建立树鼩衰老模型,为卵巢衰老的研究提供模型参考方案。 方法 ( 1) 筛选青年树鼩 8 只,随机分为对照组( n = 4)与模型组( n = 4) 。 (2)模型组按 8 g / kg 的剂量颈背部皮下注射 D-半乳糖 5 个月,采集卵巢,液氮冻存以及 4%多聚甲醛固定。 (3) HE 染色观测卵巢组织结构;免疫组织化学染色观测衰老相关标志分子蛋白表达;Ki67 染色观测增殖活性;Tunel 染色观测细胞凋亡。 结果 ( 1) 对照组树鼩卵巢组织髓质与间质界限明显,细胞排列整齐,可见原始、初级、次级和成熟卵泡,有少量闭锁卵泡;模型组树鼩卵巢组织结构散乱,局部只见原始、初级卵泡,有大量闭锁卵泡。 ( 2) 模型组衰老相关标志分子 P53、P21、P16 蛋白表达水平明显高于对照组。 (3)模型组细胞增殖活性显著低于对照组。 (4)模型组卵巢细胞凋亡率显著高于对照组。 结论 D-半乳糖可诱导卵巢组织结构散乱,卵泡闭锁,使细胞增殖活性减弱,凋亡率增加。

关键词: 树鼩, 卵巢衰老, 卵泡, 细胞凋亡, 衰老标志分子